Raj Biosis Private Limited

We are engaged in offering Bioelisa HCV 4.0 Elisa Test Bio Kit Spain to our clients. Our range of all products is widely appreciated by our clients.

BIOKIT, S.A. - Bioelisa HIV-1 2 3.0 3000-1168 96 tests 3000-1169 480 tests
ELISA test for the detection of antibodies to Human Immunodeficiency Viruses (HIV) type 1 (group M-O) or type 2 in human serum or plasma samples in clinical laboratories and as a first-line screening assay in blood centres. Summary Serological evidence of infection with HIV may be obtained by testing for presence of HIV antigens or antibodies in serum of individuals suspected for HIV infection. Antigen can generally be detected during both acute phase and the symptomatic phase of AIDS only. The antibodies to HIV-1 and/or HIV-2 can be detected throughout virtually the whole infection period, starting at or shortly after the acute phase and lasting till the end stage of AIDS. Therefore, the use of highly sensitive antibody assays is the primary approach in serodiagnosis of HIV infection. Apart from sexual transmission, the principal route of infection with HIV is blood transfusion. HIV can present both in cellular and cell-free fractions of human blood. Therefore, all donations of blood or plasma should be tested because due to the risk of HIV transmission through contaminated blood. This can be effectively achieved by testing for the antibodies to HIV-1 and HIV-2 by using a highly sensitive ELISA tests.
Principle bioelisa HIV-1 2 3.0 is a two step incubation antigen “sandwich” enzyme immunoassay kit, in which the wells of a microplate are coated with recombinant HIV antigens expressed in E. coli (recombinant HIV-1 gp41, gp120, and recombinant HIV-2 gp-36). Serum or plasma samples are added to these wells. If specific HIV1/2 antibodies are present in the sample, they will form stable complexes with the HIV recombinant antigens. The microwells are then washed to remove unbound serum proteins. A second set of recombinant antigens conjugated with peroxidase and expressing the same epitopes are added to the wells. During the second incubation step, these antigens will bind to the specific captured antibodies. After a second wash to remove unbound conjugate a Chromogen solutions are added to the wells. In wells containing the antigen-antibody-antigen “sandwich” immunocomplex, the colorless Chromogens are hydrolyzed by the bound conjugate to a blue colored product. The blue color changes to yellow after blocking the reaction with sulfuric acid. The intensity of colour can be measured and is proportional to the anti-HIV 1/2 antibodies concentration in the sample. Wells containing negative samples remain colourless.