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Elisa Kits
ELISA kits are assay systems that utilize antibodies, enzymes, and color-changing substrates to detect and quantify specific molecules in biological samples. They are available in various formats, including direct, indirect, competitive, and sandwich ELISAs, each designed for specific analytes and applications.
Applications:
Disease Diagnostics:
Description: ELISA kits are widely used in clinical diagnostics to detect biomarkers associated with diseases such as HIV, hepatitis, cancer, autoimmune disorders, and more.
Hashtags: #DiseaseDiagnostics #ClinicalELISA #MedicalTesting #Healthcare
Drug Discovery and Development:
Description: Researchers use ELISA kits in pharmaceutical and biotechnology industries to screen for potential drug candidates, assess drug efficacy, and monitor biomarker responses.
Hashtags: #DrugDiscovery #PharmaceuticalResearch #BiomarkerScreening #Biotech
Immunology Research:
Description: ELISAs are essential tools in immunology research for studying immune responses, cytokines, antibodies, and antigen-antibody interactions.
Hashtags: #ImmunologyResearch #AntigenAntibody #ImmuneResponse #ELISAAssay
Environmental Testing:
Description: ELISA kits are used for environmental monitoring, detecting contaminants, pathogens, and toxins in water, soil, and food samples.
Hashtags: #EnvironmentalTesting #ContaminantDetection #FoodSafety #WaterQuality
Biotechnology and Bioprocessing:
Description: ELISAs play a role in bioprocessing by monitoring cell culture conditions, assessing protein expression, and optimizing bioproduction processes.
Hashtags: #Bioprocessing #Biotechnology #CellCulture #ProteinExpression
Details:
ELISA kits typically include pre-coated microplates, reagents, and detailed protocols for performing specific assays. The kits are designed to provide high sensitivity and accuracy in detecting and quantifying target molecules.
Rajbiosis Oscar Elisa Test Kit
Product Brochure
Brand | Oscar |
Form | Card |
Method | Rapid |
Packaging Size | 10 Test |
Material | Plastic |
Result Time | 3 min |
Country of Origin | Made in India |
Dengue- Antigenn- ns1
hIV 3rd gen
HIV 4th Gen
Hepatitis B Elisa test kit
HCV Elisa test kit
Kala Azar Dipstickstest kit
Syphilis Elisa test kit
#ELISA
#ELISATest
#Immunoassay
#DiagnosticTest
#LabTesting
#MedicalResearch
#BiomedicalScience
#AntibodyDetection
#BiomarkerTesting
#Immunology
#MedicalLaboratory
#HealthcareTechnology
#Biotechnology
#MedicalDiagnosis
#ResearchTools
Human Anti Mullerian Hormone Amh Elisa Kit Ansh Lab
Product Brochure
ICMR Approved | Yes |
Brand | Ansh Lab |
Usage/Application | Hospital |
Packaging Type | Box |
Sensitivity | Less than 93.5pg/mL |
Detection Range | 246.91-20000pg/mL |
Country of Origin | Made in India |
Product Information
Anti-Müllerian hormone (AMH), a member of the TGFβ superfamily, is a homodimeric glycoprotein composed of two 55 kDa N-terminal and two 12.5 kDa C-terminal homodimers, non-covalently linked by disulfide bridges.1
Recent studies have shown that the AMH C-terminal homodimer is much less active than the noncovalent complex, but almost all activity can be restored by associating with the N-terminal pro-region, which reforms a complex with the mature C-terminal homodimer. This finding raises the possibility that the AMH noncovalent complex is the active form of protein.
rajbiosis j- mitra elisa test kit
Product Brochure
ICMR Approved | Yes |
Brand | J. Mitra & Co. Pvt. Ltd. |
Convenient Pack Sizes | 10 Tests, 30 Tests & 50 Tests |
Long Shelf Life | 24 Months At 2-30 Degree C |
Usage/Application | Dengue |
Packaging Type | Box |
Country of Origin | Made in India |
J- Mitra Elisa test kit-
dengue Igm Microlisa
Dengue IgG microlisa
Dengue Ns1 MIcrolisa
HCV GEn-4 Ag &Ab Microlisa
Covid 19 Ag Microlisa
Covid 19 (IgM+IgG+IgA)
covid 19 Neutralizing Antibody Microlisa
Malaria Ag Microlisa
3rd Genration HCV Microlisa
D-Dimer Microlisa
Celiac Microlisa test kit
Makesure Tsh Elisa
Minimum Order Quantity: 5 Kit
Product Brochure
ICMR Approved | Yes |
Type | Direct ELISA |
Brand | Make sure |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
Shelf Life- 1 Year
ISO 9001:2008 Certified
BoneMarrow#Myeloma#SickleCell#Hemophilia#PlateletDisorders#Hematopathology#Hemoglobinopathies#HematologyUpdates#BloodResearch#HematologyClinic#BloodCellsMorphology#HematologyStudents#HematologySymposium#BleedingDisorderAwareness#PlateletCount#HematologyDiagnosis#BloodDiseasesAwareness#HematologyTechnologist#BloodCancerResearch#HematologyConsult#HematologyCare
Additional Information:
- Delivery Time: 2-5day
- Packaging Details: Pack Size: 96 Test Shelf Life- 1 Year ISO 9001:2008 Certified
Trust wellLeptospira IgM ELISA Kit
Product Brochure
Type | Direct ELISA |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
The RecombiLISA Leptospira IgM ELISA Kit is an indirect, solid phase enzyme-linked immunosorbent assay. The two main kit components are:
1) A 96 breakable well plate with the solid microwells pre-coated with L. interrogans antigen.
2) Liquid conjugates composed of monoclonal anti-human IgM reagent conjugated with horseradish peroxidase (HRP-anti-human IgM conjugates).
This test is intended to be used for the qualitative detection of IgM anti-Leptospira interrogans (L. interrogans) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of acute infection with L. interrogans. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers.
Recommended For Use:
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Any person presenting with clinical signs or symptoms consistent with acute infection with interrogansincluding: high fever, headache, chills, muscle aches, vomiting, jaundice, red eyes, abdominal pain, diarrhea, skin rash
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After exposure to a known contaminated source
TRUSTwell HBsAg ELISA Kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
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Based on double Ab-Sandwich immunoassay technique ensure high specificity
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Solid microwells pre-coated with monoclonal anti-HBsAg antibodies
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Liquid conjugates composed of polyclonal anti-HBsAg Ab conjugated with HRP
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Relative sensitivity: 100% and specificity: 99.78 %
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Comparable accuracy to the leading other ELISA kits
-
Clear borderlines with high accuracy positive and negative controls
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Breakaway microwell strips
-
Evaluated by NIB
-
Assay time: 90 Minutes
-
12-months shelf life at 2-8 °C
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Microwells coated with anti-HBsAg Ab
-
HBsAg negative control
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HBsAg positive control
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Enzyme Conjugate
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Wash buffer (30 x concentrate)
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TMB substrate A
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TMB substrate B
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Stop solution
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ELISA Working Sheet
TRUSTwell Malaria Ag ELISA Kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
-
Target: Pf HRP II and pLDH based detection
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LOD: pLDH – 10 parasites/uL against WHO Standard
pfHPRP-II – 20 parasites/uL against FIND Standard -
Specificity: No cross-reactivity with dengue, chikungunya & filarial infection
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Precision: CV?10%
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Better sensitivity at 95% CI than competitors
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LOD is calibrated against WHO and FIND Standard
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96 well throughput suits blood donor screening
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Microwells coated with Anti-HRP II and Anti-pLDH monoclonal antibodies
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Positive control
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Negative control
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Sample Diluent
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Enzyme conjugate (50 X concen trate)
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Conjugate Diluent
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Wash buffer (30 X concentrate)
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TMB substrat e (Ready to use)
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Stop solution
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ELISA Workin g Sheet
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Product insert
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Desiccant
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Sealant
TRUSTwell Dengue IgM ELISA Kit
Product Brochure
ICMR Approved | Yes |
Sterile/ Non Sterile | Sterile |
Brand | Athenese-Dx |
Packaging Type | Box |
Usage/Application | Clinic |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
The TRUSTwell Dengue IgM ELISA Kit is a solid-phase, IgM capture enzyme linked immunosorbent assay. The three main kit components are:
1) A 96 breakable well plate with the solid microwells pre-coated with mouse monoclonal anti-human IgM antibody
2) Recombinant dengue antigen
3) HRP-anti-dengue conjugates
This test is intended to be used for the qualitative detection of IgM anti-dengue viruses (DEN1,2,3,4) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of acute dengue infection. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of healthcare providers.
Recommended For Use:
Any person presenting with clinical signs or symptoms consistent with acute dengue infection or dengue fever including: high fever, severe headaches, pain behind the eyes, severe joint and muscle pain, fatigue, nausea, vomiting, and/or skin rash.
During primary infection, anti-dengue IgM antibodies start to appear approximately 4-6 days after the onset of fever, peak at about 2 weeks, and remain in circulation for about 2-3 months. During secondary infection, IgM antibodies increase simultaneously with, or after the IgG antibody response, in general at a titer lower than IgG. Importantly, IgM antibody levels during secondary infection are significantly lower in comparison with primary infection.
RecombiLISA HEV IgM ELISA kit
Product Brochure
Type | Direct ELISA |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
-
Utilizes IgM capture technique.
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Suitable for diagnosing acute HEV infection.
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Assay time: 75 Mins.
-
Sensitivity: 100% & Specificity: 100%.
-
Utilizes IgM capture technique.
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Suitable for diagnosing acute HEV infection.
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Assay time: 75 Mins.
-
Sensitivity: 100% & Specificity: 100%.
TRUSTwell T4 ELISA Kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
A solid phase enzyme linked immunoabsorbant assay for the quantitative measurement of total Thyroxine (T4) in human serum.
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Microwells coated with anti-T4 antibody 1 plate, 96 wells
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T4 Standards: S1 (0 µg/dl) 1 mL
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T4 Standards: S2 (2.0 µg/dl) 1 mL
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T4 Standards: S3 (5.0 µg/dl) 1 mL
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T4 Standards: S4 (10.0 µg/dl) 1 mL
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T4 Standards: S5 (15.0 µg/dl) 1 mL
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T4 Standards: S6 (25.0 µg/dl) 1 mL
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HRP-T4 Conjugate Concentrate (20X) 1.2 mL
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Enzyme Conjugate Diluent 2 Vials, 7.5 mL
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Wash Buffer Concentrate (40X) 25 mL
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Substrate 12.0 mL
-
Stop solution 7.5 mL
TRUSTwell Vitamin-D (Total) ELISA kit
Product Brochure
Type | Direct ELISA |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
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Accuracy: R2 = 0.918 with CLIA as reference.
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Analytical Sensitivity: 1.2 ng/mL.
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Specificity: No cross reactivity to non-hydroxylated vitamin D2 and Vitamin D3.
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Intra & Inter assay precision: CV <=10%.
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Higher linearity: 0-150 ng/mL.
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1 step less procedure, faster resulting.
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High & Low level assay controls included.
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Accuracy: R2 = 0.918 with CLIA as reference.
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Analytical Sensitivity: 1.2 ng/mL.
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Specificity: No cross reactivity to non-hydroxylated vitamin D2 and Vitamin D3.
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Intra & Inter assay precision: CV <=10%.
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Higher linearity: 0-150 ng/mL.
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1 step less procedure, faster resulting.
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High & Low level assay controls included.
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RecombiLISA HAV IgM ELISA Test
Product Brochure
Type | Direct ELISA |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
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Utilizes IgM capture technique
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Detects IgM anti-HAV in serum or plasma
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Evaluated with BBI HAV serum conversion and HAV mixed titer performance panels
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Useful for identifying acute HAV infections
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Microwells coated with anti-human IgM antibody
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HAV IgM negative control
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HAV IgM positive control
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HRP-HAV conjugates
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Wash buffer (30 x concentrate)
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TMB substrate A
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TMB substrate B
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Stop solution
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ELISA Working Sheet
-
Product insert
TRUSTwell HCV Total Ab ELISA Kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
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Detects all IgM, IgA and IgG antibodies
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Better performance: Biotinylated antigen improves the sensitivity and specificity of the assay
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External Evaluation: NIB, Govt. Of India - Sensitivity: 100 % & Specificity: 100 %
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Internal Evaluation: Zeptometrix HCV sero-conversion panel showed early detection
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100% detection rate over market leading IgG ELISA
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Minimal assay time: 105 mins
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Shelf Life: 18 Months
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Microwells coated with recombinant HCV antigens
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HCV positive control
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HCV negative control
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Biotinylated HCV antigens(50x)
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Biotin diluent
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SA HRP Enzyme Conjugate
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Wash buffer (30 X concentrate)
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TMB substrate A
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TMB substrate B
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Stop solution
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ELISA Working Sheet
TRUSTwell Total T3 ELISA Kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
A solid phase enzyme linked immunoabsorbant assay for the quantitative determination of total Triiodothyronine (T3) levels in human serum.
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Microwells coated with T3 analog 1 plate, 96 wells
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T3 Standards: S1 (0 ng/ml) 1 mL
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T3 Standards: S2 (0.5 ng/ml) 1 mL
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T3 Standards: S3 (1.0 ng/ml) 1 mL
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T3 Standards: S4 (2.5 ng/ml) 1 mL
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T3 Standards: S5 (5.0 ng/ml) 1 mL
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T3 Standards: S6 (10 ng/ml) 1 mL
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HRP-anti-T3 Antibody Conjugates (20X Concentrate) 1.2 mL
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Enzyme Conjugate Diluent 2 vials, 7.5 mL/Ea
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Wash Buffer Concentrate (40X) 25 mL
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Substrate 12.0 mL
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Stop solution 7.5 mL
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Product insert 1 set
TRUSTwell Anti-Mullerian hormone (AMH) ELISA kit
Product Brochure
Type | Direct ELISA |
ICMR Approved | Yes |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
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Accuracy: R2 = 0.96 (with Siemens CLIA)
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Analytical Sensitivity: 0.023ng/mL
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Specificity: No-cross reactivity with other fertility hormones
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Linearity: 0 - 14ng/mL
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Intra & Inter assay precision: CV <=10%
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User friendly package: 48 Test / Kit
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Ready to use calibrators (6 level) & controls (2 level)
TRUSTwell Dengue Ag (NS1) ELISA Kit
Product Brochure
ICMR Approved | Yes |
Sterile/ Non Sterile | Sterile |
Brand | Athenese-dx |
Packaging Type | Box |
Usage/Application | Clinic |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
The TRUSTwell Dengue Ag (NS1) ELISA Kit is a solid-phase, enzyme linked immunosorbent assay that was developed using the antibody sandwich technique. The two main kit components are:
1) A 96 breakable well plate with the solid microwells pre-coated with rabbit anti- Pan Dengue NS1 antibody
2) A conjugate solution composed of antibodies against Dengue NS1 antigen conjugated with horseradish peroxidase (HRP-anti-dengue NS1 conjugates)
This test is intended for the qualitative detection of dengue NS1 antigen (DEN1, 2, 3, 4) in human serum or plasma. It is intended to be used by professionals as an aid in the early detection of dengue virus infection . Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of healthcare providers.
Recommended For Use:
Any person presenting with clinical signs or symptoms consistent with acute dengue infection or dengue fever including: high fever, severe headaches, pain behind the eyes, severe joint and muscle pain, fatigue, nausea, vomiting, and/or skin rash.
Dengue NS1 antigen is released into the blood during viral replication in an infected patient, and is detectable from the first day after the onset of fever up to Day 9. NS1 antigen can be identified before the formation of antibodies, thus making it a beneficial biomarker for early detection of dengue infection, allowing for prompt management of dengue fever.
TRUSTwell Dengue IgG ELISA Kit
Product Brochure
ICMR Approved | Yes |
Sterile/ Non Sterile | Sterile |
Brand | Athenese-Dx |
Packaging Type | Box |
Usage/Application | Clinic |
Disposable/ Reusable | Disposable |
Country of Origin | Made in India |
The TRUSTwell Dengue IgG ELISA Kit is a solid-phase, IgG capture enzyme linked immunosorbent assay. The three main kit components are:
1) A 96 breakable well plate with the solid microwells pre-coated with mouse monoclonal anti-human IgG antibody
2) Recombinant dengue antigen
3) HRP-anti-dengue conjugates
This test is intended to be used for the qualitative detection of IgG anti-dengue viruses (DEN1,2,3,4) in human serum or plasma. It is intended to be used by professionals as an aid in the diagnosis of active infection with dengue viruses, including secondary infection. Any interpretation or use of this test result must also rely on other clinical findings as well as on the professional judgment of health care providers.
Recommended For Use:
Any person presenting with clinical signs or symptoms consistent with acute dengue infection or dengue fever including: high fever, severe headaches, pain behind the eyes, severe joint and muscle pain, fatigue, nausea, vomiting, and/or skin rash.
During primary infection, anti-dengue IgG antibodies become detectable by the end of the first week of illness. They increase slowly to peak around 14-21 days, and then decrease to low levels, persisting for the duration of life. During secondary infection, a strong and rapid IgG antibody response is often observed. IgG levels are detectable as early as 3 days after the onset of symptoms, with the peak detection window being 1-2 weeks after symptom onset. In secondary infection, IgG antibodies rise to levels higher than during primary infection. Importantly, anti-dengue IgG antibodies increase earlier than or simultaneously with IgM antibodies, and at much higher levels during secondary infection.
TRUSTwell Syphilis Ab kit
Product Brochure
ICMR Approved | Yes |
Sample Type | Blood |
Brand | Athenese-Dx |
Shelf Life | 12 Months |
Country of Origin | Made in India |
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Qualitative detection of antibodies (IgG, IgM, IgA) against Treponema pallidum (Tp).
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Based on double Ag-sandwich immunoassay technique to ensure high specificity
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Solid microwells pre-coated with recombinant Tp antigens
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Liquid conjugates composed of recombinant Tp antigens conjugated with HRP
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Sensitivity: 100%, Specificity: 99.8%, Shelf life: 12 months at 2-8°C
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Assay time: 70 minutes
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Microwells coated with Tp antigen
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Syphilis negative control
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Syphilis positive control
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Enzyme Conjugate
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TMB substrate A
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TMB substrate B
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Stop solution
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ELISA Working Sheet
-
Product insert
TRUSTwell HCV IgG ELISA Kit
Product Brochure
ICMR Approved | Yes |
Type | Direct ELISA |
Usage/Application | Clinic |
Number of Reactions(Preps)/Kit | 96 well |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
-
Based on indirect immunoassay technique ensure high specificity
-
Solid microwells pre-coated with recombinant HCV antigens
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Liquid conjugates composed of anti-human IgG conjugated with HRP
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Relative Sensitivity: 100 %, Specificity: 99.81 %
-
Comparable accuracy to the leading other ELISA kits
-
Clear borderlines with high accuracy positive and negative controls
-
Breakaway microwell strips
-
Evaluated by NIB
-
Assay time: 90 Minutes
-
12-months shelf life at 2-8 °C
-
Microwells coated with recombinant HCV antigens
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Sample diluent
-
HCV negative control
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HCV positive control
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HRP–anti human IgG conjugate
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Wash buffer (30 x concentrate)
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TMB substrate A
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TMB substrate B
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Stop solution
-
ELISA Working Sheet
-
Product insert
TRUSTwell HIV Ag-Ab ELISA Kit
Product Brochure
ICMR Approved | Yes |
Type | Direct ELISA |
Brand | Athenese-Dx |
Usage/Application | Clinic |
Sterile/ Non Sterile | Sterile |
Disposable/ Reusable | Disposable |
Sample Type | Blood |
Country of Origin | Made in India |
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Based on double Ag/Ab-Sandwich immunoassay technique to ensure high specificity
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Solid microwells pre-coated with recombinant HIV-1, HIV-2 Ag and Anti-P24 Ab
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Liquid conjugates composed of recombinant HIV-1, HIV-2 Ag & SA conjugated with HRP
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Relative sensitivity: 100% and specificity: 99.9 %
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Comparable accuracy to the leading other ELISA kits
-
Clear borderlines with high accuracy positive and negative controls
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Breakaway microwell strips
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Evaluated by NIB
-
Assay time: 90 Minutes
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12-months shelf life at 2-8 °C
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Microwells coated with anti-P24 Ab, recombinant HIV-1 and HIV-2 antigen
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Biotinylated P24 Ab
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HIV Ag positive control
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HIV Ag negative control
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HIV negative control
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HRP–avidin, HIV 1+2 conjugate mixture
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Sample diluent
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Wash buffer (30 x concentrate)
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TMB substrate A
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TMB substrate B
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Stop solution
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ELISA Working Sheet
Rajbiosis J- Mitra elisa Kits Covid Kawach IgG Microlisa Chikungunya IgM Microlisa HS-CRP Quanti
Minimum Order Quantity: 2 Piece
Product Brochure
ICMR Approved | Yes |
Brand | J Mitra |
Number of Reactions(Preps)/Kit | 50 |
Result Time (Rapid Kits) | 20 min |
Sample Type | Blood |
Packaging Type | Box |
Country of Origin | Made in India |
j-mitra Elisa card:-
Covid Kawach IgG Microlisa
Chikungunya IgM Microlisa
HS-CRP Quanti Microlisa
Lepto IgM Microlisa
Scrub Typhus IgM Microlisa
Hepalisa
Hepalisa ultra
Vitamin D Quanti Microlisa
Vitamin B12 Quanti Microlisa
T3 Quanti Microlisa
T4 Quanti Microlisa
TSH Quanti Microlisa
Additional Information:
- Delivery Time: 5day
Bioelisa Hbsag 3.0 Elisa Test Bio Kit Spain
Brand | Bioelisa |
Usage/Application | Clinical |
Form | Liquid |
Method | Elisa |
Material | Reagents |
Shelf Life | 12 Months |
Result Time | 1 min |
bioelisa HBsAg 3.0
3000-1158 96 tests
3000-1159 480 tests
ELISA test for the detection of hepatitis B surface antigen (HBsAg) in human serum or plasma in clinical laboratories and as a first-line screening assay in blood centres.
Summary
Hepatitis B is a disease produced by a viral infection during which many serological markers appear. One of these markers is the HBsAg. In 1964 Blumberg and col.1 were the first to detect an antigen in the blood of an Australian aborigine which reacted with the serum antibody of a New York haemophilia patient. This antigen was later identified as hepatitis B surface antigen. The discovery and identification of hepatitis B surface antigen was a great stride forward in the knowledge and differentiation of viral hepatitis infections. The presence of HBsAg in serum or plasma constitutes the most important indicator for the diagnosis of a hepatitis B virus (HBV) infection. The enzyme immunoassay was first described by Engvall and Perlmann2 and van Weemen and Schuurs3 in 1971. The technique was subsequently developed by Voller and col.4,5 using microplates, that permitted the development of highly sensitive and accurate diagnostic kits.
Principle
bioelisa HBsAg 3.0 is a direct immunoenzymatic method of the «sandwich» type in which guinea pig anti-HBs antibodies coated to microplate wells act as the capture antibody and goat anti-HBs antibodies marked with peroxidase serve as conjugate antibodies. The sample to be analysed is incubated in one of the antibody-coated wells. If the sample contains HBsAg, the antigen will bind to the antibody on the plate. After washing to eliminate any unbound material, goat anti-HBs conjugate to peroxidase is added to the well and allowed to react with the antigen-antibody complex formed in the first incubation. After a second incubation and subsequent washing, an enzyme substrate containing a chromogen is added. The substrate will develop a blue colour if the sample is positive for HBsAg. The blue colour changes to yellow after blocking the reaction with sulphuric acid. The intensity of the colour is proportional to the amount of HBsAg in the test specimens.